In Vitro Oxidation of β-Amyrin and Erythrodiol by CYP716A12 in Microsomes of the WAT11 Strain.
(A) GC-MS analysis of the reaction products resulting from in vitro assay containing β-amyrin (1 and 2) and erythrodiol (3 and 4) as substrate on strains expressing CYP716A12 (black) and control (gray).
(B) Mass spectrum of the peaks at 26.75 min indicated by the arrows in (A): The retention time and mass spectra of these peaks compare well with those of oleanolic acid.