Figure 3.

Reliably identifying KC odor responses. A, Responses of a single KC to five presentations of 3-octanol (black traces). The blue trace shows the kinetics of the odor pulse measured by PID. Response amplitudes (inset) are calculated by averaging activity between 0.5 and 4.5 s following stimulus onset (shaded gray area). B, Responses of the same cell to the clean air control. C, Response amplitudes of the 45 strongest responding neurons from a single optical section to 3-octanol (Oct), 4-methylcyclohexanol (MCH), and clean air. Each data point is the evoked response amplitude from a single cell on a single trial. A cell was deemed responsive to the odor if it responded significantly (p < 0.01) on more than half of the trials. Responsive cells are indicated by red points, nonresponsive cells by gray. Cells are sorted independently for each stimulus according to the mean p value. D, Statistical significance of response peaks from the neurons shown in C. The y-axis shows 1 minus the p value. Cells are sorted as in C. Note that responsive cells consistently have values close to 1 on almost all trials; this is not the case for cells classified as nonresponsive.