Figure 3.
MTS-conjugated 12A10 mAb inhibits glioma cell migration. A, SF767 cells were treated with unconjugated 12A10 or MTS-conjugated 12A10 at the indicated concentration for 16 h, and the effect on migration rate was determined by radial migration assay (*, P < 0.05). B, after a migration period of 24 h, cells were fixed, permeabilized, and incubated with a FITC-tagged anti-mouse IgG. A and B, SF767 cells treated with MTS-12A10. C and D, SF767 cells treated with unconjugated 12A10. Phase contrast images are in A and C. fluorescent images are in B and D.