Table 3.
Relative expression and MALDI-TOF-MS-based identification of deregulated spots, as revealed by 2D-DIGE analysis of serum samples.
| Naive/Normal donors | Naive/IVIg donors | IVIg/Normal donors | Protein identification | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Spot no. |
Av. Ratio | t-test | Av. Ratio | t-test | Av. Ratio | t-test | Protein name | Gene name | Accession number | Matched peptides | Sequence coverage | Est'd Z score | MW (theor) | pI (theor) |
| 410 | 4.51 | 0.013 | 1.35 | 0.30 | 3.33 | 0.0018 | ||||||||
| 416 | 2.02 | 0.21 | 1.25 | 0.67 | 1.62 | 0.031 | Alpha-1-antitrypsin | SERPINA1 | P01009 | 10 | 28% | 2.34 | 44324 | 5.37 |
| 417 | 1.89 | 0.047 | 1.21 | 0.33 | 1.56 | 0.0081 | ||||||||
| 518 | 4.92 | 0.12 | 1.31 | 0.91 | 3.77 | 0.0051 | ||||||||
| 517 | 5.49 | 0.086 | 1.41 | 0.74 | 3.89 | 0.013 | Alpha-2-macroglobulin | A2M | P01023 | 17 | 17% | 2.32 | 160796 | 5.95 |
| 521 | 5.94 | 0.087 | 1.49 | 0.71 | 4.00 | 0.0082 | ||||||||
| 550 | 2.20 | 0.010 | 1.10 | 0.58 | 1.99 | 0.042 | ||||||||
| 557 | 2.47 | 0.0072 | 1.02 | 0.83 | 2.42 | 0.053 | Ceruloplasmin | CP | P00450 | 12 | 25% | 2.34 | 120085 | 5.41 |
| 558 | 2.52 | 0.0027 | −1.00 | 0.90 | 2.53 | 0.042 | ||||||||
| 564 | 7.48 | 0.11 | 1.04 | 0.78 | 7.16 | 0.013 | Alpha-2-macroglobulin | A2M | P01023 | 9 | 8% | 1.96 | 160796 | 5.95 |
| 623 | 4.27 | 0.048 | 1.07 | 0.66 | 3.99 | 0.29 | Alpha-2-macroglobulin | A2M | P01023 | 12 | 10% | 2.33 | 160796 | 5.95 |
| 666 | 7.08 | 0.0047 | 1.05 | 0.72 | 6.72 | 0.036 | ||||||||
| 668 | 2.75 | 0.043 | 1.21 | 0.57 | 2.27 | 0.035 | ||||||||
| 670 | 5.72 | 0.010 | 1.23 | 0.55 | 4.63 | 0.023 | Complement factor B | CFB | P00751 | 20 | 32% | 2.38 | 83000 | 6.66 |
| 680 | 6.20 | 0.013 | 1.32 | 0.50 | 4.71 | 0.046 | ||||||||
| 709 | 5.22 | 0.065 | 2.79 | 0.10 | 1.87 | 0.014 | ||||||||
| 795 | −1.96 | 0.022 | −1.42 | 0.061 | −1.38 | 0.033 | Afamin | AFM | P43652 | 8 | 13% | 2.25 | 66577 | 5.58 |
| 1673 | 2.61 | 0.12 | −1.41 | 0.44 | 3.68 | 0.039 | Not identified | |||||||
| 1772 | 2.53 | 0.016 | 1.30 | 0.17 | 1.94 | 0.025 | Haptoglobin | HP | P00738 | 12 | 32% | 2.35 | 43349 | 6.13 |
| 1811 | −2.32 | 0.031 | −1.55 | 0.19 | −1.50 | 0.19 | Ficolin-3 | FCN3 | O75636 | 7 | 19% | 1.95 | 30353 | 6.22 |
| 1812 | −2.52 | 0.043 | −1.42 | 0.27 | −1.78 | 0.084 | ||||||||
| 1831 | −2.75 | 0.047 | −1.53 | 0.22 | −1.79 | 0.091 | Clusterin | CLU | P10909 | 6 | 11% | 1.89 | 50062 | 5.89 |
| 1844 | −2.54 | 0.047 | −1.32 | 0.40 | −1.91 | 0.081 | ||||||||
| 1950 | −2.41 | 0.045 | −1.87 | 0.15 | −1.29 | 0.41 | Not identified | |||||||
| 2288 | −4.62 | 0.0042 | −1.72 | 0.19 | −2.68 | 0.053 | Apolipoprotein A-I | APOA1 | P02647 | 11 | 31% | 2.24 | 28078 | 5.27 |
| 2334 | −4.99 | 0.012 | −1.83 | 0.41 | −2.73 | 0.14 | Apolipoprotein A-I | APOA1 | P02647 | 14 | 43% | 2.36 | 28078 | 5.27 |
| 2605 | −3.46 | 0.013 | −1.22 | 0.43 | −2.84 | 0.011 | Not identified | |||||||