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Determination of RB and p53 dysfunction and coxsackie adenovirus receptor (CAR) expression in ATC cell lines. Western blots of 50 μg of whole-cell lysate from each ATC cell line and normal primary thyrocytes as a control were evaluated for RB (top panel), p53 (second panel), CAR (third panel) and β-actin expression as a loading control (bottom panel). The blot is representative of results obtained from three independent experiments. RB, retinoblastoma.
