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The expression of alpha smooth muscle actin was evaluated by Western blot in D3, D3N, V216 and V216L fibroblasts exposed to TGFβ1 for four days. The expression of alpha smooth muscle actin was significantly increased in D3 and V216 fibroblasts after TGFβ1 treatment. The fold change in alpha smooth muscle actin expression after TGFβ1 treatment was also significantly larger in the D3 and V216 fibroblasts when compared to the D3N and V216L fibroblasts. *p<0.05
