Table 1.
Efficiencies of DNA extraction of different amounts of cells of E. coli ATCC 11775T and S. aureus subsp. aureus ATCC 25923 by three different methods: FTA® Elute, the Reischl et al. method and QIAamp® DNA Mini Kit
Number of cells | 5.3 × 105 | 5.3 × 104 | 5.3 × 103 | 5.3 × 102 | 5.3 × 101 | |
---|---|---|---|---|---|---|
Median % (n = 4) | ||||||
E. coli ATCC 11775T | FTA® Elute | 40.5 | 84.8 | 96.3 | BCd | BCd |
Reischl et al. | 48.9 | 59.6 | 24.5 | BCb | ND | |
QIAamp® DNA Mini Kit | 4.6 | 32.3 | 13.5 | BCb | BCc | |
S. aureus subsp. aureus ATCC 25923 | FTA® Elute | 112.6 | 94.9 | 119.4 | BCb | BCc |
Reischl et al. | 10.2 | 8.8 | 8.5 | BCb | BCa | |
QIAamp® DNA Mini Kit | 69.4 | 117.0 | 97.7 | 149.9 | BCa |
ND, not detected; BC, below cut-off level, i.e. not all of the reactions were positive and quantifiable
aFour, bthree, ctwo and done of four reactions produced a positive result