Figure 9.

A375 cells apoptosis analyzed by FACScan flow cytometer. (A) Untreated A375 cells were used as controls. (B–G) Pictures are representative results from flow cytometry of propidium iodine and Annexin V-FITC-stained A375 cells treated with free DTIC, free DR5 mAb or DTIC-NPs, DTIC-NPs + DR5 mAb, DTIC + DR5 mAb, DTIC-NPs – DR5 mAb (loaded with the same amount of DTIC and DR5 mAb for 72 hours).

Abbreviations: DTIC, dacarbazine; mAb, monoclonal antibody; NPs, nanoparticles; FITC, fluorescein isothiocyanate.