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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Biol Rhythms. 2011 Oct;26(5):412–422. doi: 10.1177/0748730411414170

Figure 2.

Figure 2

The daily rhythms of protein expression and phosphorylation states in CCM mice and their WT littermates. (A) The phosphorylation of p42/44 ERK was rhythmic in WT mice (n = 5), in which diphosphorylated ERK (pERK) peaked at ZT 0. In CCM mice (n = 5), pERK was no longer rhythmic. (B) The phosphorylation of p38 kinase was rhythmic in both WT (n = 3) and CCM mice (n = 3). *Phosphorylated p38 at ZT 12 was significantly higher than ZT 0, 6, and 18 in WT mice. #Phosphorylated p38 at ZT 12 was significantly higher than ZT 6 in CCM mice. (C) The phosphorylation of AKT at thr308 (pAKTthr308) peaked at ZT 6 in WT mice (n = 3), while the diurnal rhythm of pAKTthr308 was dampened in CCM mouse (n = 3) hearts. (D) In WT mouse hearts (n = 4), the phosphorylation of GSK3β (pGSK) was significantly higher at ZT 18 compared to ZT 0 and 6, while there was no diurnal rhythm of pGSK in CCM mice (n = 3).