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GC trace of Mtb chloroform:methanol extracts. Lipids from Mtb cells grown to mid-log phase were extracted in chloroform:methanol and analyzed by GC/MS to detect low-molecular weight compounds. (A) Trace showing all species detected by GC/MS. (B) GC trace showing only those species containing ions characteristic of Mtb diterpenes (ions 80, 189 and 191). Chemical structures of diterpenes are adapted from previous studies [7,17] (Fig. S1).
