Table 1.

Localization and relative abundance of Mtb diterpenes. γ-irradiated Mtb cells (Colorado State University) were separated into cell wall, cell membrane and cytosol fractions, and lipids were extracted using hexane (for cell wall) or chloroform:methanol (for cytosol and cell membrane). Culture filtrate prepared from live Mtb cultures was lyophilized, and lipids were extracted in chloroform:methanol. Tuberculosinol, edaxadiene and edaxadiene B were detected by GC/MS and the relative abundances are indicated.

Fraction	Tuberculosinol	Edaxadiene	Edaxadiene B
Cell membrane	+	+++	++
Cell wall	−	+	−
Cytosol	−	−	−
Culture filtrate	−	+	−