. Author manuscript; available in PMC: 2011 Dec 8.

Published in final edited form as: Structure. 2010 Dec 8;18(12):1667–1677. doi: 10.1016/j.str.2010.09.019

Table 1.

N-lobe dimer interface mutations affect PknB autophosphorylation

PknB Mutant	Calculated Mass (Da)	Observed Masses (Da)^a	PO₄ sites
PknB-FKBP	47,875.0	48,399.0 (avg. of 6P & 7P)^b ^c	ND^d
Arg10Ala-FKBP	47,789.9	48,186 (5P), 48,113 & 48,273 (4P & 6P), 48,045 & 48,376 (3P & 7P)	ND
Leu33Asp-FKBP	47,876.9	48,114.9 (3P), 48,033.0 (2P)	T171, T173, T294
Asp76Ala-FKBP	47,831.0	48,067.2 (3P), 47,988.0 (2P)	T171, T173, T294
Asp138Asn-FKBP	47,874.0	47,873.1 (0P)	none
Asp138Asn_1-308	33,478.7	33,480.4 (0P)	none

The species are listed in order of abundance, and the number of phosphates (P) is indicated in parentheses.

The species with molecular mass 48,399.0 (representing an average of 6–7 phosphates) was the most abundant species.

A molecular mass of 48,350.0 (6P) was the lowest molecular weight species observed.

ND - not determined in this study.