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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Methods Mol Biol. 2011;714:71–82. doi: 10.1007/978-1-61779-005-8_5

Figure 2.

Figure 2

RNA and Protein Colocalization in Xenopus Oocytes. Stage III oocytes were injected with Alexa-546-labeled VLE RNA and cultured to allow localization of the injected RNA. Immunofluorescence was subsequently performed using antibodies directed against PTB/hnRNP I, an RNA binding protein that associates with VLE RNA and is required for VLE RNA localization (6). Vegetal localization of VLE RNA is shown in the red channel (A), and PTB distribution is visualized in the green channel (B). Co-localization of VLE RNA and PTB protein is evident in the overlay of the red and green channels, and is shown in yellow (C). Scale bar = 50 μm.