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. Author manuscript; available in PMC: 2011 Sep 27.

Published in final edited form as: Biochem Pharmacol. 2009 Sep 6;79(3):330–338. doi: 10.1016/j.bcp.2009.09.003

(A) Cellular uptake of curcumin and curcumin (NP). KBM-5 cells were incubated with Hoechst (50 ng/mL) for 30 min, and then washed two times with PBS, resuspended in media and then incubated with 10 µM curcumin or curcumin (NP). The cells were harvested at different time intervals and the cellular uptake (green fluorescence) was monitored by fluorescence microscopy as described in methods. (B) Disappearance of curcumin and curcumin (NP) from the cells stained with Hoechst (50 ng/mL) for 30 min. KBM-5 (1×10⁶) cells were incubated with 10 µM curcumin or curcumin (NP) for 30 min, washed two times with PBS and again incubated at 37°C. The cells were harvested at different time intervals and the cellular uptake was determined by fluorescence microscopy as described in methods.

(A) Cellular uptake of curcumin and curcumin (NP). KBM-5 cells were incubated with Hoechst (50 ng/mL) for 30 min, and then washed two times with PBS, resuspended in media and then incubated with 10 µM curcumin or curcumin (NP). The cells were harvested at different time intervals and the cellular uptake (green fluorescence) was monitored by fluorescence microscopy as described in methods. (B) Disappearance of curcumin and curcumin (NP) from the cells stained with Hoechst (50 ng/mL) for 30 min. KBM-5 (1×10⁶) cells were incubated with 10 µM curcumin or curcumin (NP) for 30 min, washed two times with PBS and again incubated at 37°C. The cells were harvested at different time intervals and the cellular uptake was determined by fluorescence microscopy as described in methods.