. Author manuscript; available in PMC: 2011 Sep 27.

Published in final edited form as: Mutat Res. 2007 Aug 7;637(1-2):161–172. doi: 10.1016/j.mrfmmm.2007.08.001

Table 1.

Comparative Mutagenesis of γ-HOPdG, Reduced γ-HOPdG, and Reduced γ-HOPdG-Mediated KWKK Crosslink in COS-7 Cells^*.

DNA modification	Correct sequence	Mutations at the modified site, number				Mutations at positions adjacent to the modified site, number				Mutations at the modified site, %	Overall sequence alterations^**, %
DNA modification	Correct sequence	G to A	G to C	G to T	Deletion	5′C to A	5′C to G	5′C to T	3′A deletion	Mutations at the modified site, %	Overall sequence alterations^**, %
non- damaged dG	229	0	0	0	0	0	0	0	0	0.0	0.0
γ-HOPdG	299	1	2	8	0	1^***	0	0	2	3.5	4.2
reduced γ-HOPdG	245	0	0	2	0	0	0	3	2	0.8	2.8
reduced γ-HOPdG-mediated KWKK crosslink	311	2	9	17	1	2^****	1^***	2	1	8.4	9.6

Site-specifically modified vectors were constructed using either 12-mer or 30-mer inserts.

^**

To calculate percentage, mutations in tandem were counted as one sequence alteration.

^***

tandem with adducted G to T.

^****

1 out of two mutations was in tandem with adducted G to C.