Figure 1. Pharmacological inhibition of SMS causes extensive tubulation of the TGN.

HeLa cells were treated with DMSO (control for PKD inhibitor) (1A) or water (control for SMS inhibitor) (1C), and 20 µM of PKD inhibitor (1B) or 30 µg/ml of SMS inhibitor D609 (1D). After 4 hours of incubation, cells were fixed and processed for indirect Immunofluorescence with anti TGN 46 (trans golgi marker) rabbit polyclonal antibodies and stained with anti rabbit Alexa Fluor® 488-conjugated secondary antibodies. Confocal images were captured and processed using an LSM 510 META. Images are representatives of at least three independent experiments. Dashed boxes outline the areas that were magnified.