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. 2011 Sep 27;6(9):e24990. doi: 10.1371/journal.pone.0024990

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Hu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Trans 2K (TransGen Biotech, Beijing, China) molecular marker is shown in the first lane. Primer pair PRCmon-F/PRCmon-R were used to produce a 354 bp fragment (lanes 2–6) from all five Monilinia species, but not from other closely related genera (Botrytinia,lane 7; Sclerotinia, lane 8). Reverse primer Mon-R and species-specific forward primers Ensis-F, Cola-F, and Mume-F, produced amplicons 237 bp (lane 2), 534 bp (lane 3), or 712 bp (lane 4) in length from M. yunnanensis, M. fructicola, or M. mumecola, respectively.