Figure 6. ROS inhibition reverses hypoxia-stimulated HIF-1α accumulation and nuclear translocation in MCF-7 cells.

After pretreatment with 2 mM NAC, 10 µM LY294002 or 10 µM U0126 for 1 h, cells were grown under hypoxia for 2 h and stained for HIF-1α (red) as described in ‘Materials and methods’. Cells were counterstained with DAPI (blue). Images are representative of at least 3 independent determinations. The illustrations in the right side column show the merged images of HIF-1α and DAPI. Magnification, ×400.