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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Bone. 2011 Apr 29;50(2):568–575. doi: 10.1016/j.bone.2011.04.017

Figure 2. Daily injections of osteocalcin increase insulin secretion and β-cell mass in wild-type mice.

Figure 2

Analyses were performed in mice injected daily with the indicated doses of osteocalcin (OCN) or vehicle for 16 weeks. (A) Glucose-stimulated insulin secretion test. The calculated area under the curve is represented in the histogram on the right. (B) Insulin immunostaining (counterstained with hematoxylin) showing larger islets in the pancreas of mice injected daily with osteocalcin (magnification 100X, scale bar: 200 μm). (C) Histomorphometric comparisons of β-cell mass, β-cell area and islet number. #: p<0.05, ##: p<0.01 when comparing Vehicle and OCN 3 ng/g/day groups. *: p<0.05, **: p<0.01 when comparing Vehicle and OCN 30 ng/g/day groups. Six to 15 mice per group were analyzed.