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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Bone. 2011 Apr 29;50(2):568–575. doi: 10.1016/j.bone.2011.04.017

Figure 3. Daily injections of osteocalcin improve glucose tolerance and insulin sensitivity in mice fed a high-fat diet.

Figure 3

Analyses were performed in mice fed a normal diet (ND) or a high-fat diet (HFD) for 8 weeks and then injected daily with 30 ng/g/day of osteocalcin (OCN) or with vehicle for the indicated time. (A) Blood glucose after 6 h fasting. (B) Glucose tolerance test performed after 8 weeks of treatment. (C) Insulin tolerance test performed after 8 weeks of treatment (Insulin injection: 1.5 U/kg). For B and C the calculated area under the curve is represented in histograms on the right. (D) Fasting serum insulin level. (E) HOMA-IR (see Material and Methods). #: p<0.05, ##: p<0.01, ###: p<0.001 when comparing HFD + Vehicle and HFD + OCN groups. *: p<0.05, **: p<0.01, ***: p<0.001 when comparing to ND + Vehicle group. Five to 8 mice per group were analyzed.