Table 1. Active site motifs in human members of the PlsC superfamily that have GPAT or AGPAT activity.

	Motif I catalysis /acyl-CoA binding	Motif II G-3- P/LPA binding	Motif III G-3-P binding	Motif IV acyl-CoA binding	Motif V	Accession Number	Alias
GPAT1	PVHRSHIDY	LGGFFIRR	FLEIFLEGTRSRSGKTSCARAG	ILIIPVGISY		Q9HCL2	Gpam, mtGPAT
GPAT2	STHKTLLDS	RACSPALR	LEPPGALGPRLSALGQAWVGFV	ALLVPVAVTY		Q6NUI2.2	xGPAT70
GPAT3	ANHTSPIDV	CPHVWFER	PILIFPEGTCINNTSVMMFKKG	GTIHPVAIKY	VWYMPPMTREEGED	Q53EU6.2	See Table 2
GPAT4	ANHTSPIDV	CPHVWFER	PILIFPEGTCINNTSVMMFKKG	ATVYPVAIKY	VWYLPPMTREADED	Q86UL3.1	See Table 2
GNPAT	PSHRSYIDF	SGAFFMRR	PVEFFLEGTRSRSAKTLTPKFG	TYLVPISISY		O15228.1	DHAPAT
AGPAT1	SNHQSSLDL	AGVIFIDR	RVWVFPEGTRNHNGSMLPFKRG	QAQVPIVPIV	VLPPVPTEGLTPDD	Q99943.2	LPAATα
AGPAT2	SNHQSILDM	GGVFFINR	KVWIYPEGTRNDNGDLLPFKKG	QAQVPIVPVV	VLEAIPTSGLTAAD	O15120.1	LPAATβ
AGPAT3	LNHNFEIDF	TVVEGLRR	WFLLYCEGTRFTETKHRVSMEV	YHLLPRTKGF		Q9NRZ7.1

Bolded and underlined amino acids are believed to be critical for function, based on site-directed mutagenesis and/or mutational analysis in E. coli,^29–30 and mouse GPAT⁴²⁷ or human AGPAT1,⁸² and by analysis of human disorders caused by deficiencies of AGPAT2,^36–37 dihydroxyacetone phosphate acyltransferase (GNPAT),⁴²⁸ and tafazzin, which is a 1-palmitoyl-2-[¹⁴C]linoleoyl-phosphatidylcholine:monolysocardiolipin linoleoyltransferase.^35,429