Table 2. Comparison of fold change values of 20 genes (with P≤0.05) selected from the mRNA-seq platform to qRT-PCR and cDNA microarray.
Gene symbol | Gene Name | mRNA-seq data (P≤0.05) | qRT-PCR (Fold change) | cDNA microarray (Fold change) | ||
Affecteda | Unaffecteda | Fold change | ||||
AFAP1 | Actin filament associated protein 1 | 75.1 | 37.5 | 2.00 | 1.97 | 1.94 |
CCRL1 b | Chemokine (C-C motif) receptor-like 1 | 0.19 | 22.8 | −119.3 | −98.3 | −1.87 |
CHPF | Chondroitin polymerizing factor | 142.3 | 231.9 | −1.63 | −1.80 | −1.59 |
CRIP1 | Cysteine-rich intestinal protein 1 | 39.0 | 95.0 | −2.44 | −6.32 | −1.81 |
DKK2 | Dickkopf homolog 2 | 0.16 | 2.81 | −18.1 | −17.3 | −2.30 |
DYNC1I1 | Dynein, cytoplasmic 1, intermediate chain 1 | 0.02 | 0.56 | −30.8 | −35.9 | −1.08c |
EMP1 | Epithelial membrane protein 1 | 99.4 | 217.2 | −2.18 | −2.06 | −2.72 |
EXT1 | Exostosin 1 | 184.3 | 104.3 | 1.77 | 1.95 | 1.55 |
FAM20A | Family with sequence similarity 20, member A | 0.27 | 7.48 | −27.7 | −32.2 | −2.04 |
FBLN5 | Fibulin-5 | 73.1 | 172.8 | −2.36 | −2.60 | −2.03 |
GPR160 | G protein-coupled receptor 160 | 0.78 | 0.02 | 41.8 | 1.37 | 1.08c |
HTR1B b | 5-hydroxytryptamine (serotonin) receptor 1B | 0.87 | 0.01 | 62.0 | 4.15 | 1.51 |
KIF23 | Kinesin family member 23 | 601.9 | 2.03 | 295.9 | 1.17 | 1.30c |
LRRK2 | Leucine-rich repeat kinase 2 | 0.05 | 1.30 | −27.1 | −29.8 | n/a |
LXN | Latexin | 10.7 | 0.10 | 111.4 | 166.3 | 2.22 |
MEST | Mesoderm-specific transcript homolog | 83.6 | 4.56 | 18.3 | 36.7 | 1.80 |
RAP1B b | Ras-related protein 1b | 78.9 | 39.1 | 2.02 | 1.46 | −1.21c |
RASGRP1 | RAS guanyl releasing protein 1 (calcium and DAG-regulated) | 19.1 | 0.47 | 40.3 | 35.7 | 17.3 |
SOD3 b | Superoxide dismutase 3, extracellular | 6.44 | 81.1 | −12.6 | −25.1 | −1.44 |
STC2 | Stanniocalcin 2 | 149.3 | 85.9 | 1.74 | 1.90 | 1.52c |
values based on RPKM normalization;
one qRT-PCR probe used;
not significant.