Table 1. Transcripts encoding proteins of central metabolism that are present in altered abundance after shifting anaerobic cultures of E. coli MG1655 to micro-aerobic (10 µM O2) conditions.
| *Operon name | Product | †Fold change in abundance relative to the anaerobic steady-state at the indicated times (min) following the switch to micro-aerobic conditions | ‡Relevant regulatory proteins | ||||
| 5 | 10 | 30 | 60 | 120 | |||
| acnB | aconitase B | 2.3 | 4.1 | 3.1 | 3.3 | ArcA (−) | |
| adhE | alcohol dehydrogenase | 0.43 | 0.27 | 0.42 | 0.24 | 0.32 | FNR (+) |
| appBC | cytochrome bdII | 0.30 | 0.24 | AppY (+) ArcA (+) | |||
| aspA | aspartase | 0.37 | 0.26 | 0.22 | 0.19 | 0.21 | FNR (+) |
| cyoA-E | cytochrome bo oxidase | 25.1 | 27.2 | 29.6 | 31.1 | 29.6 | ArcA (−) FNR (−) PdhR (−) |
| fdoGHI-fdhE | formate dehydrogenase | 4.3 | 3.8 | 4.7 | |||
| focA-pflB | formate transport/PFL | 0.50 | 0.42 | 0.48 | 0.4 | ArcA (+/−) FNR (+) | |
| frdABCD | fumarate reductase | 0.44 | FNR (+) | ||||
| fumC | fumarase | 2.1 | 3.8 | 3.2 | 4.0 | ArcA (−) | |
| gltA | citrate synthase | 0.27 | ArcA (−) | ||||
| hyaA-F | hydrogenase 1 | 0.21 | 0.14 | ArcA (+) | |||
| hycA-I | hydrogenase 3 | 0.42 | 0.19 | 0.06 | |||
| hypA-E | hydrogenase 3 accessory proteins | 0.4 | 0.33 | 0.26 | FNR (+) | ||
| narGHIJ | nitrate reductase | 0.48 | 0.13 | 0.22 | 0.14 | 0.29 | FNR (+) |
| ndh | NADH dehydrogenase II | 3.9 | 5.7 | 7.5 | 5.4 | 5.3 | ArcA (−) FNR (−) PdhR (−) |
| nirB-D-cysG | nitrite reductase | 0.36 | 0.23 | 0.2 | 0.11 | 0.13 | FNR (+) |
| nrfA-F | nitrite reductase | 0.33 | 0.13 | 0.08 | 0.05 | 0.06 | FNR (+) |
| pdhR-aceEF-lpdA | PDHC | 4.0 | 4.5 | 4.0 | 3.5 | 2.7 | FNR (−/+) PdhR (−) |
| sdhCDAB | succinate dehydrogenase | 13.4 | 12.1 | 3.8 | 2.2 | ArcA (−/+) FNR (−) | |
| sucABCD | succinyl-CoA synthetase | 4.1 | |||||
| yfiD | PFL repair protein | 7.1 | 9.2 | 12.0 | 6.5 | 5.1 | ArcA (+) FNR (+/−) PdhR (−) |
*The data shown are for the first gene in the operon.
†
Fold change (by at least 2-fold at one or more time-points) is the ratio of transcript levels at the indicated time to the transcript levels at t = 0 (the initial anaerobic state; p≤0.05).
‡
Relevant regulatory proteins are indicated (−) denotes negative regulation, (+) positive regulation, and (−/+) negative andtab positive regulation. The Table was compiled from references in the text and EcoCyc database [17]. Cells with no entry indicate no significant change in transcript abundance.