Figure 4.

Mitochondrial volume is maintained even in the presence of distribution deficits. Three-dimensional volume reconstructions (A) of 8 × 70 nm sections immunofluorescently stained for VDAC in 8.5-month-old nonTg (left) and Tg4510 (right) mice were used to measure volumes of individual mitochondria in the neuropil. These volume measurements were verified by comparison with volumes estimated from cross-sectional measurements of mitochondria on electron microscopy images (B). Each mitochondrion was measured at its widest and longest points as demonstrated with lines within the mitochondria beside the arrows. An ellipsoidal volume approximation was calculated from these diameters. Mitochondrial volumes approximated from electron micrographs confirmed the mitochondrial volumes obtained with the automated output of the array tomography method. Quantification of array volumes of n = 3922 mitochondria demonstrated no significant differences in volumes of individual mitochondria of the neuropil (C) between 8.5-month-old Tg4510 and nonTg mice (depicted as medians with SD). Scale bar = 2 μm.