Skip to main content
. 2011 Sep 27;6(9):e25610. doi: 10.1371/journal.pone.0025610

Figure 5. IFN-γ and IL-17 production.

Figure 5

Concentration of (a) IFN-γ, and (b) IL-17 in PBMC cell culture supernatants stimulated with wild-type Streptococcus pneumoniae strain (WT), and an isogenic pneumolysin (Ply)-deficient mutant and media only from healthy controls (grey bars) (n = 6) and HIV-infected subjects (white bars) (n = 9). Data are mean values ± standard error of the mean. (c) Representative flow cytometric data of media only, HIV- and HIV+ T cells producing IFN-γ and IL-17 following WT CCS stimulation. CFSElow pneumococcal-specific proliferating T cells were selectively gated and cytokines evaluated. (d) CFSElow pneumococcal specific T cell IFN-γ and IL-17 production following WT and (e) Ply-CCS in HIV- (black circles n = 11) and HIV+ (white circles n = 13−17) individuals. Grey data points represent pneumococcal carriers (nasopharynx). Black horizontal bars represent median values of total antigen specific responses above background. Statistical significance was analyzed by the Mann Whitney U test.