Table 1. Primers and probes used in quantitative real-time RT-PCR.
Target gene | 5′-Primer | TaqMan probe | 3′-Primer |
Acc1 | TGTCCGCACTGACTGTAACCA | TCTTCCTCAACTTTGTGCCCACGGTC | TGCTCCGCACAGATTCTTCA |
Fasn | CCTGGATAGCATTCCGAACCT | CCTGAGGGACCCTACCGCATAGC | AGCACATCTCGAAGGCTACACA |
Scd1 | CAACACCATGGCGTTCCA | TGACGTGTACGAATGGGCCCGA | GGTGGGCGCGGTGAT |
Elovl6 | GCGCTGTACGCTGCCTTTAT | TCGGCATCTGATGAACAAGCGAGC | GCGGCTTCCGAAGTTCAAA |
Srebp1 | CCAGAGGGTGAGCCTGACAA | CAATCAGGACCATGCCGACCTCT | AGCCTCTGCAATTTCCAGATCT |
Srebp2 | GCGGACAACACACAATATCATTG | AGCGCTACCGGTCCTCCATCA | TGACTAAGTCCTTCAACTCTATGATTTTG |
Ppara | TGGTTCCTGGTGCCGATTTA | TGGTGGTAGATGCCTGCAACCCCA | ACTAGCATCCCACTTAATTATGTATCTGAA |
LXRa | CGACAGAGCTTCGTCCACAA | CGGAAAAAGGGCCCAGCCCC | GCTCGTTCCCCAGCATTTT |
FXR | CACGAAGATCAGATTGCTTTGC | CAAAGGGTCCGCAGTGGAGGCC | CCGCCGAACGAAGAAACA |
Mttp [Mm00435015_m1] | * | * | * |
Apob [Mm01545156_m1] | * | * | * |
Timp3 [Mm00441827_m1] | * | * | * |
TACE [Mm00456428_m1] | * | * | * |
β2MG | CTGATACATACGCCTGCAGAGTTAA | GACCGTCTACTGGGATCGAGACATGTG | ATGAATCTTCAGAGCATCATGAT |
*Not given; commercially acquired from Life Technologies Corporation, Carlsbad, CA.