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Double immunofluorescence of pimonidazole (HP) binding and neurons (NeuN) (A) and astrocytes (GFAP) (B) at day 7 after infection. NeuN labels neuronal nuclei and the perinuclear area. Arrows denote pronounced co-localization of areas in which both neurons and pimonidazole are present. When staining for astrocytes, co-localization is not observed. Scale bars: 25 μm.
