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. 2011 Oct;179(4):1951–1960. doi: 10.1016/j.ajpath.2011.06.034

Figure 1.

Figure 1

Clinical appearance of the keloid of patient 1 and cartilage oligomeric matrix protein (COMP) mRNA and protein expression in the keloid tissues. Microarray analysis was performed using RNAs extracted from keloid-derived fibroblasts (KDFs) derived from the 23 × 7.5-cm keloid and from NDFs derived from the surrounding uninvolved skin on the chest of a 68-year-old Japanese male (patient 1 in Table 1) (A). (B) COMP mRNA amount was examined by quantitative real-time RT-PCR, as mentioned in Materials and Methods. Statistical difference was determined by the Student's t-test. **P < 0.01 (C) Skin tissue from the keloid and uninvolved (normal) skin from the five patients (patients 1 to 5 in Table 1) were homogenized in 20 mmol/L HEPES, pH 7.2, containing 1% Nonidet P-40, 0.4 M NaCl, and aprotinin and were analyzed for COMP and β-actin levels by Western blot. The lower graph shows mean ± SD of COMP/β-actin protein ratios of patients 1 to 5 (n = 5). Statistical difference was determined by the Student's t-test. *P < 0.05.