Table 1.

Tumor Spectrum in Mutant Tp53 Rats

Rat ID	Genotype	Sex	Age (weeks)	Tumor diagnose	IHC⁎	LOH	Metastases
60	−/−	Male	18	Hemangiosarcoma	Factor VIIIra+++	—	Lung
				B-cell lymphoma	CD3+; CD79a+++	—	Liver
61	−/−	Male	18	Hemangiosarcoma†	Factor VIIIra+++	—	Lung
62	−/−	Male	18	Hemangiosarcoma†	Factor VIIIra++	—	Lung
66	−/−	Male	20	Adrenocortical carcinoma	NSE+; Factor VIIIra-; Synaptophysin-; Chromogranin A-	—	Lung
76	−/−	Male	26	Hemangiosarcoma†	ND	—	Lung
77	−/−	Male	17	ND‡		—	—
140	−/−	Female	22	No tumor detected	—	—	—
164	−/−	Male	13	Hemangiosarcoma	Factor VIIIra+++; Pankeratin-; Vimentin+++	—	—
198	−/−	Male	13	Rhabdomyosarcoma; undifferentiated sarcoma	ND	—	—
199	−/−	Male	14	Hemangiosarcoma	ND	—	—
223	−/−	Female	15	Hemangiosarcoma	ND	—	Lung
9	+/−	Female	36	Rhabdomyosarcoma	MyoD1++; Factor VIIIra+	Yes	Lung
20	+/−	Female	45	Osteosarcoma	ND	Yes	ND
63	+/−	Male	50	Sarcoma; transitional cell carcinoma in situ	ND	Yes	—
74	+/−	Male	45	No tumor detected	—	—	—
75	+/−	Male	40	ND‡	—	ND	—
82	+/−	Male	45	Osteosarcoma	ND	Yes	Lung
97	+/−	Male	47	Osteosarcoma	ND	Yes	Lung
100	+/−	Female	45	Osteosarcoma	ND	Yes	—
126	+/−	Female	31	No tumor detected	—	—	—

ND, not determined; LOH, loss of heterozygosity.

^⁎Immunohistochemistry used to determine tumor type. +++ indicates frequent expression of indicated marker, ++ indicates moderate expression, + means rare marker expression, and — means no marker expression found.

^†Primary tumor could not be identified; however multiple pulmonary metastases were found and used for IHC analyses.

^‡Animal was found dead in the cage and in a decomposing state.