Figure 2.

Centrosomal fragmentation induced by Poloxin is partially rescued by Kiz T379E. A: Working schedule. To obtain sufficient mitotic cells for analysis, HeLa cells, transfected, selected, and synchronized, were treated for 10 hours with a lower concentration of 15 μmol/L Poloxin, because 25 μmol/L Poloxin detaches HeLa cells, treated with transfection, selection, and synchronization, from the cell culture slides, along with increasing the mitotic cell population. B: HeLa cells were treated as described in A and stained for Plk1, pericentrin, α-tubulin, and DNA. Cells treated with DMSO were taken as control (top row). Scale bar = 5 μm. C: Evaluation of centrosomal fragmentation in approximately 300 mitotic cells from each group in B. The results are presented as mean ± SD. con, control. D: Expression levels of Myc-tagged wild-type Kiz and its variants in HeLa cells.