Table 1.
Antibodies Used in the Present Study
| Antibody | Type | Specificity or epitope | Label | Host | Concentration or dilution | Supplier |
|---|---|---|---|---|---|---|
| HT7 | Monoclonal | Human protein tau | Biotin | Mouse | IF, 0.55 μg/mL | Innogenetics |
| AT8 | Monoclonal | P-Ser202/P-Thr205 | Biotin | Mouse | IHC, 0.56 μg/mL | Innogenetics |
| MHCII | Polyclonal | Activated microglia | NA | Rat | IF, 0.5 μg/mL IHC, 0.05 μg/mL | Pharmingen |
| CD11b | Monoclonal | Mouse complement type 3 receptor | NA | Rat | IF, 1 μg/mL | Serotec |
| CD45 | Polyclonal | Mouse CD45 leukocyte common antigen, Ly-5 | NA | Rat | IF, 0.125 μg/mL IHC, 0.06 μg/mL | Pharmingen |
| A2 mol/L | Polyclonal | Mouse α2-macroglobulin | NA | Rabbit | IHC, 1/10,000 | Nordic |
| Immunoglobulins | Polyclonal | Mouse IgG, IgA, IgM | HRP | Goat | IHC, 2 μg/mL | Dako |
| IgM | Polyclonal | IgM μ-chain–specific | Biotin | Goat | IHC, 1 μg/mL | Vector |
| Albumin | Polyclonal | Mouse albumin | HRP | Goat | IHC, 0.05 μg/mL | Bethyl |
| Transferrin | Polyclonal | Mouse transferrin | HRP | Goat | IHC, 0.1 μg/mL | Bethyl |
| CD31 | Monoclonal | Mouse PECAM-1 | NA | Rat | IHC, 0.015 μg/mL | Pharmingen |
| PH2AXS139 | Polyclonal | Human phospho-histone H2A | NA | Rabbit | IHC, 0.5 μg/mL | Abcam |
Abcam, Inc., Cambridge, MA; Bethyl Laboratories, Inc., Montgomery, TX; Dako Corp., Carpinteria, CA; Immunogenetics, Inc., Buena, NJ; Nordic Immunologic Laboratories, Eindhoven, The Netherlands; BD Biosciences Pharmingen, Inc., San Diego, CA; Serotec, Inc., Raleigh, NC; Vector Laboratories, Inc., Burlingame, CA.
HRP, horseradish peroxidase; IF, immunofluorescence; NA, not available.