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. 2011 Oct;179(4):2108–2119. doi: 10.1016/j.ajpath.2011.06.017

Figure 2.

Figure 2

PTEN is phosphorylated at the serine 380 site in PEL. RT-PCR was performed to determine PTEN mRNA expression in PEL; representative gels are shown for PTEN (A) and GAPDH (B). NT, nontemplate control. C: Immunoblots were performed on protein isolated from whole-cell lysate; representative immunoblots are shown for total PTEN and phospho-PTEN (S380). β-actin was used as loading control. Equivalent amounts of protein were loaded in each case, except for HEK293, which served to indicate the molecular weight of PTEN.