Table 3.
Expression Levels of miRNAs in Human Pituitary Adenomas
| miRNA | Expression level (mean ± SD) and significance⁎ |
|||
|---|---|---|---|---|
| NFA | GH | PRL | ACTH | |
| miR-127-3p | 0.559 ± 0.569 | 1.767 ± 1.279 | 0.482 ± 0.22 | 0.348 ± 0.143 |
| versus normal | P = 0.06 | P = 0.178 | P = 0.028 | P = 0.006 |
| versus NFA | P = 0.054 | P = 0.417 | P = 0.048 | |
| miR-134 | 0.047 ± 0.190 | 5.281 ± 2.712 | 1.44 ± 0.794 | 0.951 ± 0.926 |
| versus normal | P < 0.001 | P = 0.006 | P = 0.289 | P = 0.896 |
| versus NFA | P = 0.002 | P = 0.017 | P = 0.041 | |
| miR-136 | 0.002 ± 0.01 | 1.492 ± 0.884 | 0.932 ± 0.972 | 0.138 ± 0.26 |
| versus normal | P < 0.001 | P = 0.195 | P = 0.885 | P < 0.001 |
| versus NFA | P = 0.005 | P = 0.108 | P = 0.348 | |
| miR-154 | 0.087 ± 0.140 | 2.163 ± 0.706 | 0.512 ± 0.125 | 0.309 ± 0.217 |
| versus normal | P < 0.001 | P = 0.004 | P < 0.001 | P < 0.001 |
| versus NFA | P < 0.001 | P < 0.001 | P = 0.057 | |
| miR-299-5p | 0.048 ± 0.127 | 4.692 ± 2.359 | 1.003 ± 0.215 | 0.492 ± 0.295 |
| versus normal | P < 0.001 | P = 0.006 | P = 0.993 | P = 0.003 |
| versus NFA | P = 0.002 | P < 0.001 | P = 0.012 | |
| miR-323-3p | 0.029 ± 0.111 | 2.345 ± 1.108 | 0.737 ± 0.576 | 0.828 ± 0.591 |
| versus normal | P < 0.001 | P = 0.018 | P = 0.415 | P = 0.543 |
| versus NFA | P = 0.002 | P = 0.068 | P = 0.017 | |
| miR-329 | 0.041 ± 0.090 | 2.422 ± 0.944 | 0.57 ± 0.262 | 0.5 ± 0.296 |
| versus normal | P < 0.001 | P = 0.006 | P = 0.02 | P = 0.006 |
| versus NFA | P = 0.001 | P = 0.012 | P = 0.009 | |
| miR-337 | 1.356 ± 1.234 | 4.262 ± 4.593 | 0.26 ± 0.582 | 0.395 ± 0.68 |
| versus normal | P = 0.212 | P = 0.109 | P = 0.043 | P = 0.061 |
| versus NFA | P = 0.166 | P = 0.005 | P = 0.007 | |
| miR-369-5p | 0.047 ± 0.071 | 2.328 ± 0.707 | 0.254 ± 0.352 | 0.29 ± 0.32 |
| versus normal | P < 0.001 | P = 0.002 | P = 0.005 | P < 0.001 |
| versus NFA | P < 0.001 | P = 0.314 | P = 0.123 | |
| miR-369-3p | 0.08 ± 0.188 | 2.107 ± 1.273 | 0.747 ± 0.57 | 0.127 ± 0.17 |
| versus normal | P < 0.001 | P = 0.062 | P = 0.394 | P < 0.001 |
| versus NFA | P = 0.006 | P = 0.061 | P = 0.635 | |
| miR-370 | 0.290 ± 0.270 | 4.004 ± 2.353 | 0.707 ± 0.182 | 0.676 ± 0.278 |
| versus normal | P < 0.001 | P = 0.015 | P = 0.074 | P = 0.068 |
| versus NFA | P = 0.006 | P = 0.004 | P = 0.016 | |
| miR-376c | 0.039 ± 0.102 | 2.382 ± 0.931 | 0.442 ± 0.27 | 0.271 ± 0.245 |
| versus normal | P < 0.001 | P = 0.007 | P = 0.013 | P = 0.001 |
| versus NFA | P = 0.001 | P = 0.033 | P = 0.064 | |
| miR-377 | 0.067 ± 0.132 | 4.058 ± 1.403 | 1.072 ± 0.334 | 0.619 ± 0.291 |
| versus normal | P < 0.001 | P = 0.001 | P = 0.699 | P = 0.023 |
| versus NFA | P < 0.001 | P = 0.002 | P = 0.002 | |
| miR-380 | 1.496 ± 1.174 | 3.095 ± 2.5 | 1.101 ± .556 | 0.806 ± 0.315 |
| versus normal | P = 0.119 | P = 0.069 | P = 0.754 | P = 0.399 |
| versus NFA | P = 0.187 | P = 0.148 | P = 0.007 | |
| miR-410 | 0.036 ± 0.174 | 4.285 ± 1.936 | 1.122 ± 1.208 | 1.104 ± 0.835 |
| versus normal | P < 0.001 | P = 0.004 | P = 0.835 | P = 0.767 |
| versus NFA | P = 0.001 | P = 0.134 | P = 0.019 | |
| miR-431 | 2.441 ± 2.240 | 6.157 ± 3.569 | 1.585 ± 1.055 | 1.876 ± 0.833 |
| versus normal | P = 0.007 | P = 0.009 | P = 0.291 | P = 0.033 |
| versus NFA | P = 0.042 | P = 0.135 | P = 0.186 | |
| miR-432 | 0.067 ± 0.198 | 4.247 ± 1.582 | 1.253 ± 0.734 | 1.213 ± 0.671 |
| versus normal | P < 0.001 | P = 0.001 | P = 0.511 | P = 0.486 |
| versus NFA | P < 0.001 | P = 0.029 | P = 0.006 | |
| miR-433 | 0.065 ± 0.098 | 0.979 ± 0.985 | 0.402 ± 0.331 | 0.539 ± 0.309 |
| versus normal | P < 0.001 | P = 0.958 | P = 0.011 | P = 0.007 |
| versus NFA | P = 0.061 | P = 0.131 | P = 0.011 | |
| miR-770-5p | 3 ± 3.704 | 2.95 ± 2.301 | 0.984 ± 0.67 | 1.248 ± 1.027 |
| versus normal | P = 0.018 | P = 0.067 | P = 0.963 | P = 0.566 |
| versus NFA | P = 0.928 | P = 0.013 | P = 0.035 | |
⁎
Values from normal pituitaries were designated as 1, against which values from pituitary tumors were normalized. Data were obtained using 10 normal pituitaries, 25 NFAs, and 7 GH-secreting, 5 PRL-secreting, and 7 ACTH-secreting tumors. P values were obtained from two-tailed Student's t-test with unequal variance.