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. 2011 Oct;179(4):1681–1692. doi: 10.1016/j.ajpath.2011.06.013

Figure 12.

Figure 12

Enhanced renal tissue expression of EMT markers by Vpr-Agt-4. A: Total RNA was extracted from renal tissues of Vpr-Agt-4 and Vpr-Agt-2 (protocol C). RNA was probed for α-SMA and ZEB2 by real-time PCR studies. Vpr-Agt-4 showed enhanced renal tissue expression of α-SMA and ZEB2. *P < 0.01 compared with respective Vpr-Agt-2. B: Protein was extracted from renal tissue of Vpr-Agt-2 (n = 3) and Vpr-Agt-4), Western blots were prepared and probed for α-SMA. Cumulative data are shown in the form of a bar diagram. Representative gels showing expression of α-SMA and actin are also shown. *P < 0.01. C: Protein was extracted from renal tissue of Vpr-Agt-2 (n = 3) and Vpr-Agt-4 (n = 3), Western blots were prepared and probed for E-cadherin. Cumulative data are shown in the form of a bar diagram. Representative gels showing expression of E-cadherin and actin are also shown.