Table 2.

List of Antibodies Included in the Protein Pathway Array

Antibodies specific for phosphorylation

p-PKCα(Ser657), p-EGFR (Tyr1068), p-HER2/ERBB2 (Tyr1221/1222), p-PDK1 (Ser241), p-PKCα/βII (Thr638/641), p-p53 (Ser392), p-Akt (Ser473), p-PTEN (Ser380), p-Rb (Ser780), p-survivin (Thr 34), p-beta-catenin (Ser33/37/Thr41), p-STAT5 (Tyr694), p-STAT3 (Ser727), p-ERK (Thr202/Tyr204), p-p70 S6 kinase (Thr389), p-VEGFR-2 (Tyr951), p-FGFR (Tyr653/654), p-EIF4B (Ser422), p-HGFR/C-Met (Y1234/Y1235), p-Smad (Ser463/465), p-ERK5 (Thr218/Tyr220), p-p90RSK (Ser380), p-CREB (Ser133), p-mTOR (Ser2448), p-PKCδ(Thr505), p-CDC2 (Tyr15), p-c-Jun (Ser73), p-SAPK/JNK (Thr183/Tyr185), p-FLT3 (Tyr 591), p-p38(Thr180/Tyr182), p-GSK-3α/β(Ser21/9), p-FAK (Tyr397), p-RB (Ser807/811), p-HGFR/C-Met (Y1003).

Antibodies for signal transduction proteins

CyclinB1, cyclinD1, CDK6, CDC25B, cyclinE, CDK2, p27, BRCA1, CDK4, neu, 14-3-3 beta, cPKCα, ERK, EGFR, WEE1, CDC25C, HSP90, CHK1, MDM2, CDC2 p34, E2F-1, PCNA, c-myc, Notch1, beta-catenin, Akt, Trap, XIAP, Bcl-2, ETS1, HIF-1α, HIF-2α, TTF-1, p53, Notch4, PTEN, SRC-1, p300, c-Kit, Bax, N-cadherin, Raf-1, CDC42, EIF4B, TNF-α, vimentin, OPN, survivin, E-cadherin, TGF-β, p16, p27, WT1, Mesothelin, Cleaved Caspase-3, COX2, ATF-1, CREB, p21, NF-κB52, NF-κB50, calretinin, H-Ras, Bcl-6, K-Ras, alpha-tubulin, NF-κB p65, Myf-6, p15, ATR, Fas, SUMO-1, MetRS, Ep-CAM, FOXM1, Era, SYK, STAT1, Eg5, HIF-3α, RAD52, ATM, ABCG2, Bad-7, KLF6, CaMKKa, Topo IIa, p38, IL-1β, TERT, Ub, PR, Rap1, HCAM, Lyn, twist, TAP, patched, Erb,VEGF, GLI-3, FGF-7, p63, SK3, rhoB, WNT-1, TDP1, SLUG.

Underlines indicate detectable expression in either tumor or normal tissues.

All phosphorylation state-specific antibodies were obtained from Cell Signaling Technology (Danvers, MA), except p-HGFR/C-Met (Y1234/Y1235) and p-HGFR/C-Met (Y1003), which were purchased from R&D Systems (Minneapolis, MN).

All non-phospho-antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA), except the following antibodies: 1) ERK, Akt, beta-catenin, Notch4, CREB, Cleaved Caspase-3, EIF4B, NF-κB52, NF-κB50, and STAT1 were obtained from Cell Signaling Technology (Danvers, MA); 2) XIAP was obtained from BD Biosciences (San Jose, CA); 3) TGF-β was obtained from R&D Systems (Minneapolis, MN).

TFG, transforming growth factor.