. 2011 Jul 9;33(11):2217–2223. doi: 10.1007/s10529-011-0682-9

Table 1.

Eight SSRs, primer sequences, and allele size based on the original sequenced isolate P6497

Locus	Super-contig^a	Repeat motif^b	Primer sequences (5′–3′)	Actual size isolate P6497 (bp)	GeneBank accession no.
PS01	9	(GACACT)₄₉	F: TGATGGGAGATGGCTACAGG	419	EF667485
PS01	9	(GACACT)₄₉	R: TCGCAACGACAGATTGATG	419	EF667485
PS05	3	(TCAG)₃₄	F: GAAACAATCAACCGAACAACG	263	EF667486
PS05	3	(TCAG)₃₄	R: ATAGGAGGGCAAACTGGATG	263	EF667486
PS10	NI^c	(CAAAC)₂₇	F:CGACGAAGAACAACATTACTTG	228	EF667489
PS10	NI^c	(CAAAC)₂₇	R: ATGAAACCGAACCAAACCTG	228	EF667489
PS12	32	(GCTGTT)₂₃	F: GCTGCTTGTTGCTGTTGTTG	306	EF667490
PS12	32	(GCTGTT)₂₃	R: GCGGGTGTTTGGAGAGTATC	306	EF667490
PS16	20	(ATTAT)₂₀	F: AATCTGACTTGGACGCTGTG	469	EF667491
PS16	20	(ATTAT)₂₀	R: GCTTAGTGTTTTGGGTTACGC	469	EF667491
PS24	36	(CT)₁₆ +(CT)₃	F: GTCATTTCCCTCGCTCACAC	252	EF667495
PS24	36	(CT)₁₆ +(CT)₃	R: ACACTGGCAACAAGCAACAG	252	EF667495
PS29	NI	(TAC)₁₅	F: CCACTGAAGCGAGGTAGAGG	273	EF667499
PS29	NI	(TAC)₁₅	R: GTAGCACAAAATCCGTCTGC	273	EF667499
PS33	2	(AT)₁₅	F: CTGCTAGTGCCGTTCGTTG	267	EF667501
PS33	2	(AT)₁₅	R: TAAAAGGGCTGCTCAAATCG	267	EF667501

^aTyler et al. (2006) and Dorrance and Grundwald (2009)

^bSuper-contig or DNA region in the P. sojae genome where the microsatellite is located

^c‘+’ indicates that two simple repeats were separated by other bases

^d NI not identified