Table 2.
Loci, primers, labeling method and alleles detected in this study (page 1 of 2)
| Locus | Primer | Gel method | Applied biosystems 3730 genetic analyzer (ABI)b | CEQ 8000 genetic analysis system (CEQ)d | Allele name | Difference in bp between ABI and CEQ |
|---|---|---|---|---|---|---|
| Approximate band size (bp)a | Allele size (bp)c | Allele size (bp)e | ||||
| PS01 | PS01-F | – | n/df | 206 | PS01-B | – |
| 257 | n/d | 260 | PS01-C | – | ||
| PS01-R | – | n/d | 266 | PS01-D | – | |
| 269 | 269 | 272 | PS01-E | 3 | ||
| 281 | 281 | 284 | PS01-F | 3 | ||
| – | 287 | 290 | PS01-G | 3 | ||
| 293 | 293 | 296 | PS01-H | 3 | ||
| – | n/d | 418 | PS01-I | – | ||
| 419 | 419 | 424 | PS01-A | 5 | ||
| – | 425 | 430 | PS01-J | 5 | ||
| – | 431 | 436 | PS01-K | 5 | ||
| – | 437 | 442 | PS01-L | 5 | ||
| – | 443 | 448 | PS01-M | 5 | ||
| PS05 | PS05-F | – | n/d | 260 | PS05-B | – |
| 263 | 263 | 264 | PS05-A | 1 | ||
| PS05-R | – | n/d | 268 | PS05-C | – | |
| 290 | n/d | 296 | PS05-D | – | ||
| – | n/d | 304 | PS05-E | – | ||
| 307 | 307 | 308 | PS05-F | 1 | ||
| – | n/d | 312 | PS05-G | – | ||
| – | n/d | 316 | PS05-H | – | ||
| – | n/d | 320 | PS05-I | – | ||
| 335 | 335 | 336 | PS05-J | 1 | ||
| – | 339 | 340 | PS05-K | 1 | ||
| – | 343 | 344 | PS05-L | 1 | ||
| – | 347 | n/d | PS05-M | – | ||
| 360 | 355 | n/d | PS05-N | – | ||
| PS10 | PS10-F | – | n/d | 144 | PS10-B | – |
| 153 | 146 | 149 | PS10-C | 3 | ||
| PS10-R | – | 191 | 194 | PS19-D | 3 | |
| 198 | 196 | 199 | PS10-E | 3 | ||
| – | 211 | 214 | PS10-F | 3 | ||
| 218 | n/d | 219 | PS10-G | – | ||
| – | 221 | 224 | PS10-A | 3 | ||
| 228 | n/d | 229 | PS10-H | – | ||
| 233 | n/d | 234 | PS10-I | – | ||
| 238 | n/d | 249 | PS10-J | – | ||
| – | 251 | n/d | PS10-K | – | ||
| PS12 | PS12-F | 258 | 251 | 252 | PS12-B | 1 |
| 264 | 257 | 258 | PS12-C | 1 | ||
| PS12-R | – | 292 | 293 | PS12-D | 1 | |
| 300 | 298 | 299 | PS12-E | 1 | ||
| – | 304 | 305 | PS12-F | 1 | ||
| 306 | 310 | 311 | PS12-A | 1 | ||
| – | 316 | 317 | PS12-G | 1 | ||
| PS16 | PS16-F | – | 395 | 403 | PS16-B | 8 |
| 405 | 405 | 413 | PS16-C | 8 | ||
| PS16-R | – | n/d | 418 | PS16-D | – | |
| – | 455 | 463 | PS16-E | 8 | ||
| – | 460 | n/d | PS16-F | – | ||
| – | n/d | 478 | PS16-G | – | ||
| 470 | 470 | 483 | PS16-A | 13 | ||
| – | 475 | 488 | PS16-H | 13 | ||
| PS24 | PS24-R | 236 | 236 | 238 | PS24-B | 2 |
| 252 | 252 | 254 | PS24-A | 2 | ||
| PS24-F | 262 | 262 | 264 | PS24-C | 2 | |
| – | n/d | 310 | PS24-D | – | ||
| – | n/d | 338 | PS24-E | – | ||
| PS29 | PS29-R | 252 | 249 | 251 | PS29-B | 2 |
| PS29-F | 273 | 270 | 272 | PS29-A | ||
| PS33 | PS33-R | – | 250 | 252 | PS33-B | 2 |
| 257 | 256 | 258 | PS33-C | 2 | ||
| PS33-F | – | 258 | 260 | PS33-D | 2 | |
| – | 262 | 264 | PS33-E | 2 | ||
| – | 264 | 266 | PS33-F | 2 | ||
| 267 | 266 | 268 | PS33-A | 2 | ||
| – | 268 | 270 | PS33-G | 2 | ||
| – | 270 | 272 | PS33-H | 2 | ||
| – | 274 | 276 | PS33-I | 2 | ||
| – | 278 | 280 | PS33-J | 2 |
aTotal number of isolates analyzed n = 190; 126 from Ohio and 64 from Iowa
bForward primer PS01, PS16, PS24, and PS33 were labeled with FAM while PS05, PS10, PS12, and PS29 were labeled with HEX dye
cTotal number of isolates analyzed n = 106; 93 isolates from Iowa (includes 64 isolates used for the gel method) and 13 isolates received from Ohio’s lab. Size is based on approximate band sizes as determined by GeneMapper® Software 4.0
dAll forward primer were designed with a M13(−21) tail at the 5′-end and used a universal WellRED D4 labeled M13(−21) primer as a nested primer, except for primers PS01 and PS05 that were directly labeled using WellRED D4
eTotal number of isolates analyzed n = 130; 126 isolates from Ohio (same isolates used for the gel method) and 4 isolates received from Iowa’s lab. Size is based on approximate band sizes as determined by CEQ 8000 genetic analysis software
fNot detected in the isolates from that laboratory
Alleles found on the isolates shared by laboratories (n = 17) are italicized
Allele corresponding to sequenced isolate P6497 is bolded