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. 2011 Aug 31;30(17):3662. doi: 10.1038/emboj.2011.309

Figure 5.

Figure 5

Localization of ABP140 mRNA is dependent on the length of the ORF. (A) Amino acids 18–67 are not specifically required for mRNA localization. FISH against ABP140 mRNA in cells where amino acids 18–67 of ABP140 were replaced by GFP. In the control strain, GFP is inserted between amino acids 17 and 18. ABP140 mRNA localization to the distal pole is not affected in these strains. (B) FISH against GFP in cells expressing (1–17)-GFP, (1–17)-4GFP, (1–67)-GFP, or (1–67)TAA–GFP. The exposure time for the quadruple GFP construct was reduced to compensate for the increase in signal strength. If the actin-binding domain is appended with four copies of GFP instead of one, mRNA localization to the distal pole is recovered. If a stop codon is inserted between ABP140(1–67) and GFP, mRNA localization to the distal pole is lost. (C) Quantitation of (A, B). Sketches of the constructs are included. See Figure 1B and 2B for details on the representation. The white bars in (A, B) correspond to 5 μm.