Figure 7.

Deletion of Jarid2 does not affect the expression of epidermal differentiation genes, but reduces the genomic occupancy of PRC2 in the p16/INK4a locus. (A) The transcript level of Cdkn2a/p16 mRNA is elevated in basal epidermal keratinocytes isolated from 8-week-old mice compared with their wt counterparts. (B) Immunohistochemical analysis showing increased expression of p16 in the interfollicular epidermis and hair follicles in the backskin of Jarid2 cKO adult mice compared with their control littermates. Scale bars: 100 and 20 μm, respectively. (C) Jarid2 and Suz12 colocalize on the promoter of the p16/INK4a locus. ChIP was performed on primary mouse keratinocytes isolated from 8-week-old Jarid2 cKO and control mice (n=5 for each). Data are presented as percentage of the input.