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. Author manuscript; available in PMC: 2011 Sep 28.
Published in final edited form as: Cancer. 2007 Jul 15;110(2):420–431. doi: 10.1002/cncr.22781

FIGURE 2.

FIGURE 2

In vitro tumor cell migration assays. The migration of tumor cells was assessed by counting the number of cells that migrated through transwell. The cells that migrated through the membranes into the lower wells were assessed by hematoxylin uptake and the number of cells was counted, or by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. (A) Representative figures of cell migration in WF-0 and WF-3 cells. (B) Bar graph depicting total number of tumor cells that migrated through transwell. Note that the cell numbers that migrated through the membranes in the WF-3 group were significantly higher than those in the WF-0 group (P < .05, 1-way analysis of variance [ANOVA]). (C) Bar graph depicting absorbance at 570 nanometers (nm) using enzyme-linked immunoadsorbent assay (ELISA) in WF-0 or WF-3 tumor cells treated with MTT solution. Note that the absorbance at 570 nm of the WF-3 group were also significantly higher than those of the WF-0 group (P < .05, 1-way ANOVA).