Figure 1. Time-dependent distribution of lipid droplets in mouse jejunum after an olive oil gavage.

At 4 h after food withdrawal, mice received or not (control) an olive oil bolus (150 μl) by gavage. Control mice were killed 5 h after food withdrawal (A–D) and mice that received a lipid bolus were killed 1 h (E–H) or 20 h (I–L) after gavage. Jejunum was processed for lipid staining by the imidazole-buffered osmium tetroxide method and examined by optical microscopy after Toluidine Blue staining (A, E, F, I; scale bar, 5 μm) or by electron microscopy (B–D, G, H, J–L; scale bar, 2 μm). CLDs are indicated by arrows at the optical microscopy level and labelled as CLDs at the electron microscopy level. Red asterisks label the Golgi apparatus and N indicates the nuclei.