Figure 5. Intracellular distribution of ApoA-IV in Caco-2/TC7 cells.

Cells were cultured on semi-permeable filters for 3 weeks and then supplied with lipid micelles for 24 h. (A) Western-blot analysis of ApoA-IV distribution in sucrose gradient fractions prepared from differentiated Caco-2/TC7 cells as described in Figure 3. (B) Cells were labelled for ApoA-IV (red) and calnexin (green). Empty arrowheads indicate co-localizations. Nuclei were stained with DAPI (blue). Scale bar, 10 μm. (C) Cells were labelled for ApoA-IV (red), PLIN-2 (fuchsia), neutral lipids (green) and nuclei (blue). Scale bar, 5 μm. The boxed area in the merged Figure is shown magnified on the right. White and empty arrowheads indicate PLIN-2 and ApoA-IV labelling respectively at the surface of lipid droplets.