Figure 6. Immunoelectron microscopy analysis of ApoA-IV localization in Caco-2/TC7 cells.

Cells were cultured on semi-permeable filters for 3 weeks and then supplied with lipid micelles for 24 h. After fixation, cells were embedded in LR White resin and ultrathin sections were incubated with anti-ApoA-IV antibodies followed by 18 nm gold particle-labelled secondary antibody. (A) Low magnification of the apical part of a cell (note the cell microvilli at the top left corner of the Figure). The lipid droplets appear as electron-lucent structures. Scale bar, 500 nm. The boxed areas in (A) are shown enlarged in (B–D). Note that the gold particles gather in small clusters around the edges of the lipid droplets (arrowheads).