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Involvement of NADPH oxidase in ROS generation. Note that hypoxia-induced stimulation was reversed by a NADPH oxidase inhibitor. The fluorescence intensity of DCF-DA in ASCs was decreased by 100 nM DPI treatment in Facs analysis (A, the first peak, negative control; the second peak, normoxia; the third peak, hypoxia; the forth peak, DPI treatment). Hypoxia-induced proliferation (B) and migration (C) of ASCs were reversed by DPI treatment. *p<0.05, **p<0.01.
