FIG. 6.

Demonstration of functional vessels in vivo using Matrigel plug. (A) Matrigel plug with hiPSC-ECs were implanted via subcutaneous injection in the dorsal region of 8-week-old SCID mice. The plugs were harvested 2 weeks later. (B) (I) HE stain of Matrigel plug. (II, III) Some of these microvessels have mouse blood cells in their lumen (arrow). (C) 14-day Matrigel plugs were stained with anti-human CD31 (red) and anti-CD144 (green) antibodies, showing microvessels that are immunoreactive with these human-specific antibodies. Color images available online at www.liebertonline.com/scd