Table 1.

List of primers used in the qPCR validation assays

Gene	Forward sequence	Reverse sequence	Amplicon size bp
GAPDH	CAAACCGTTGGTGACAGTATTTG	GCACTGAGCATAAGGACACATCTAA	69
RHO_57	ATCCCTTTCCTCTCAGGAAAAAA	TCAAGTCAGCCCACTACAGAGTTG	95
RHO 60	CCCCGAGGGCATGCA	TGTAAGTGGCGAACATGTAGATGA	95
USP-like	TGACTCACAGCGCCACATG	TGAGAGAAGCCAAAAAAAGC	71
Tr_22897	TGCGCTGGAACGAAGATG	GCCCTGAGGTCCGAAGCT	60
PLA2G12B	TCTCTCCTCAGCATGTGAAACG	AGGGTCTGCAGCCCAGAGT	71
GLUT1	GTCATGTAACCCTGATGAAAAGGA	CGCGCCGCAGACTTTCT	60
C2	AGGTGCTCACTGCGCTTACA	AAGTGCAACCGCTGTGCAT	70
CXCR1	CCCTGAGCACTGCTTTGTTTT	GGTTGACGCAGCTGTGGAA	60
KCNK12	CCACGGTCGCCCACAT	ACAATCTGCGGCCCTCAGTA	67
PCYT1B	CCTCCGTCCCTGAGCAGAA	TGGCGTAGGACAGCATTCG	64

GADPH (glyceraldehyde 3-phosphate dehydrogenase): reference gene used for normalization of the qPCR results. Target genes were RHO_57 (rhodopsin transcript variant ENSGACT-00000003757), RHO_60 (rhodopsin transcript variant ENSGACT00000003760), USP-like (ubiquitin specific protease-like), Tr_22897 (transcript ‘ENSGACT00000022897’), PLA2G12B (phospholipase 2 group XIIB), GLUT1 (glucose transporter 1), C2 (complement precursor), CXCR1 (interleukin-8 receptor alpha), KCNK12 (potassium channel), and PCYT1B (phosphate cytidylyltransferase 1, choline, beta).