Table 5.
qPCR results of microarray data validation
| Gene | Type | Fold change | STD error | P value | Regulation |
|---|---|---|---|---|---|
| GAPDH | reference | 1.0 | |||
| RHO_57 | target | 12.1 | 0.573 | 0.038 | up |
| RHO 60 | target | 6.4 | 0.463 | 0.006 | up |
| USP-like | target | 5.9 | 0.443 | 0.002 | up |
| Tr_22897 | target | 1.5 | 1.231 | 0.25 | NS |
| PLA2G12B | target | 4.0 | 0.784 | 0.207 | NS |
| GLUT1 | target | 0.5 | 0.354 | 0.006 | down |
| C2 | target | 0.6 | 0.452 | 0.03 | down |
| CXCR1 | target | 0.3 | 0.139 | 0.002 | down |
| KCNK12 | target | 1.2 | 0.784 | 0.312 | NS |
| PCYB12 | target | 2.7 | 1.289 | 0.046 | up |
GADPH (glyceraldehyde 3-phosphate dehydrogenase): reference gene used for normalization of the expression values of the target genes. Target genes were RHO_57 (rhodopsin transcript variant ENSGACT00000003757), RHO_60 (rhodopsin transcript variant ENSGACT00000003760), USP (ubiquitin-spe-cific protease), Tr_22897 (transcript ‘ENSGACT00000022897’), PLA2G12B (phospholipase 2 group XIIB), GLUTl (glucose transporter 1), C2 (complement precursor), CXCR1 (interleukin-8 receptor alpha), KCNK12 (potassium channel), PCYT1B (phosphate cytidylyltransferase 1, choline, beta). Fold changes of the target genes are expression changes relative to the expression of the reference gene, which is by default 1. The direction of the changes in gene expression is indicated in the ‘Regulation’ column, up are upregulated genes, down are downregulated genes and NS represents genes whose expression is not statistically different from the reference gene.