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. 2011 Sep 26;208(10):2017–2031. doi: 10.1084/jem.20110750

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© 2011 Josefsson et al.

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Figure 1. — Thrombocytosis in the absence of Bak and Bax. (a) Western blot of Bak^−/− Bax^Pf4Δ/Pf4Δ cell lysates demonstrating efficient deletion of Bak and Bax in platelets, and cultured megakaryocytes (MGKs) derived from murine BM. Actin served as a control for protein loading. (b) Platelet counts in Bak^−/− Bax^fl/fl, Bak^−/− Bax^Pf4Δ/Pf4Δ, Bak^+/+Bax^−/−, and control mice at 7 wk of age. Data represent mean ± SEM. Each symbol represents an individual mouse. (c) Platelet survival curves in Bak^−/− Bax^fl/fl, Bak^−/− Bax^Pf4Δ/Pf4Δ, and Bak^+/+Bax^−/− mice. Platelets were labeled via intravenous injection of a DyLight 488–conjugated anti-GPIbβ (CD42c) antibody. Data represent mean ± SD. n = 4–6 mice per group. (d) Morphologically recognizable MGKs in H&E-stained sections of BM from Bak^−/−Bax^fl/fl, Bak^−/− Bax^Pf4Δ/Pf4Δ, Bak^+/+ Bax^−/−, and control mice. Each symbol represents the mean number per field of view (200×) from 8–10 fields per individual mouse. Data represent overall mean ± SEM. (e) MGK ploidy distribution profiles in adult conditional knockout mice. Data represent mean ± SD. n = 8 mice per genotype. (f) Serum thrombopoietin levels in adult conditional knockout mice. Each symbol represents an individual mouse. Data represent mean ± SD. **, P < 0.005; ***, P < 0.0001.