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. 2011 Sep 26;208(10):2017–2031. doi: 10.1084/jem.20110750

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© 2011 Josefsson et al.

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Figure 4. — Megakaryocytes require Bcl-x_L for survival during platelet formation. Proplatelet formation (a) and viability (b) of cultured fetal liver–derived MGKs harboring a constitutive or conditional deletion of Bcl-x. Data represent mean ± SEM. Bcl-x^+/+, n = 7; Bcl-x^−/−, n = 8; Bcl-x^fl/fl, n = 18; and Bcl-x^Pf4Δ/Pf4Δ, n = 13 technical replicates. n = 3 biological replicates for Bcl-x^−/−; n = 5 for Bcl-x^Pf4Δ/Pf4 and corresponding controls. (c) Representative still images from time-lapse video microscopy of cultured fetal liver-derived Bcl-x^fl/fl (Video 1) and Bcl-x^Pf4Δ/Pf4Δ (Video 2) MGKs. Frames were captured every minute for 24 h. Videos run at 25 frames/s. Videos start at 10.7 h (Video 1) and 1.3 h (Video 2). Time (h). Bar, 100 µm. (d) Representative still images from time-lapse video microscopy of cultured fetal liver–derived Bcl-x^−+/+ (Video 3) and Bcl-x^Pf4Δ/Pf4Δ (Video 4) MGKs binding Alexa Fluor 488–conjugated Annexin V (green). Frames were captured every 15 min for 24 h. Videos run at 6 frames/s. Time (h). Bar, 100 µm.