Table I.
Blood counts in mice lacking Bcl-xL in the megakaryocyte lineage
| Bcl-x fl/fl | Bcl-x +/fl | Bcl-x +/Pf4Δ | Bcl-x Pf4Δ/Pf4Δ | |
| n = 12 | n = 16 | n = 20 | n = 20 | |
| Platelets (×106/ml) | 1,139 ± 150 | 1,163 ± 170 | 697 ± 100b | 22 ± 4b |
| aPlatelets (×106/ml) | 939 ± 92 | 52 ± 28b | ||
| MPV (femtoliters) | 7.7 ± 0.6 | 7.1 ± 0.9 | 7.3 ± 0.8 | 14.0 ± 2.5b |
| PDW (%) | 53.2 ± 4.2 | 51.2 ± 5.6 | 52.3 ± 7.1 | 78.9 ± 20.9b |
| Erythrocytes (×109/ml) | 10.7 ± 0.4 | 10.7 ± 0.5 | 10.7 ± 0.5 | 10.1 ± 0.6 |
| Hematocrit (%) | 50.8 ± 1.7 | 51.1 ± 2.2 | 51.4 ± 2.3 | 49.1 ± 2.2 |
| Leukocytes (×106/ml) | 10.5 ± 1.7 | 9.4 ± 2.0 | 10.2 ± 2.4 | 9.1 ± 1.8 |
| Neutrophils (×106/ml) | 0.8 ± 0.2 | 0.8 ± 0.1 | 0.9 ± 0.2 | 0.8 ± 0.2 |
| Lymphocytes (×106/ml) | 9.1 ± 1.5 | 8.0 ± 1.8 | 8.8 ± 2.3 | 7.6 ± 1.6 |
| Monocytes (×106/ml) | 0.2 ± 0.1 | 0.2 ± 0.0 | 0.2 ± 0.1 | 0.3 ± 0.1 |
| Eosinophils (×106/ml) | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 |
Peripheral blood cell counts from mice at 7 wk of age. MPV, mean platelet volume; PDW, platelet distribution width. Data represent mean ± SD.
a
Platelet counts in whole blood determined by flow cytometry with Sphero blank calibration particles (3.5–4.0 µm), gating on CD41+ve and TER-119−ve cells. n = 3 mice per genotype. Unpaired Student’s t test with two-tailed p-values.
b
P < 0.001, with respect to control mice Bcl-xfl/fl or Bcl-x+/fl, 1 way ANOVA with Bonferroni’s multiple comparison correction.